2'-OMe-Bz-C Phosphoramidite

2′-OMe-Bz-C Phosphoramidite is a modified phosphoramidite monomer that may be utilized in the preparation and synthesis of oligonucleotides. As a 2′‑O‑methyl ribocytidine derivative, 2′-OMe-Bz-C Phosphoramidite incorporates two key modifications into RNA sequences: the 2′‑O‑methyl group enhances nuclease resistance and thermal stability of RNA duplexes, while the N⁴‑benzoyl (Bz) group protects the exocyclic amine of the cytosine base during synthesis. In therapeutic oligonucleotide applications, 2′-OMe-Bz-C Phosphoramidite is a standard building block for the synthesis of 2′‑O‑methyl RNA oligonucleotides with improved pharmacokinetic properties. The 2′‑O‑methyl modification of 2′-OMe-Bz-C Phosphoramidite is also used to study RNA structure, RNA‑protein interactions, and the effects of chemical modifications on RNA function. 2′-OMe-Bz-C Phosphoramidite is fully compatible with standard automated RNA synthesizer coupling protocols.

Product Parameters

Product Advantages

1. Enhanced Nuclease Resistance

The 2′‑O‑methyl group in 2′-OMe-Bz-C Phosphoramidite confers significant resistance to nuclease degradation, making oligonucleotides synthesized with this building block suitable for in vivo applications where metabolic stability is critical — a key advantage for antisense and siRNA therapeutics.

2. Increased RNA Duplex Thermal Stability

2′‑O‑methyl modifications increase the melting temperature (Tm) of RNA duplexes, enhancing binding affinity to complementary RNA targets. This property improves the potency and specificity of antisense oligonucleotides and siRNAs synthesized using 2′-OMe-Bz-C Phosphoramidite.

3. Standard Coupling Protocol Compatibility

2′-OMe-Bz-C Phosphoramidite is fully compatible with standard automated RNA synthesizer coupling protocols, requiring no special modifications to the synthesis cycle — enabling straightforward incorporation into existing RNA synthesis workflows.

4. High Purity for Reproducible Results

Cosperpharm supplies 2′-OMe-Bz-C Phosphoramidite at ≥95% purity (up to 99% for high‑grade material), with batch‑specific characterization data to support reproducibility in RNA oligonucleotide synthesis and biological assays.

5. Widely Used 2′‑O‑Methyl Building Block

The 2′‑O‑methyl modification is one of the most extensively characterized and widely used RNA backbone modifications in therapeutic oligonucleotides. 2′-OMe-Bz-C Phosphoramidite has been validated in numerous preclinical and clinical studies for antisense, siRNA, and aptamer applications.

Storage Conditions

Store 2′-OMe-Bz-C Phosphoramidite in a tightly sealed container under inert atmosphere (nitrogen or argon) at 4 °C for short‑term storage, or at –20 °C for long‑term storage. Protect from light and keep the container dry. The compound is moisture‑sensitive; therefore, desiccated storage is strongly recommended. Allow the sealed container to reach ambient temperature before opening to minimize moisture condensation.

Shelf life: 12 months when stored at 4 °C in a sealed container under inert atmosphere, protected from light; longer storage at –20 °C recommended for extended shelf life.

Handling precautions: Use only in a fume hood. Wear chemical‑resistant gloves, safety goggles, and a lab coat. Avoid generating dust or aerosols. Do not eat, drink, or smoke in work areas. After handling, wash hands thoroughly. Refer to the Safety Data Sheet (SDS) for complete safety information.

Quality Assurance at Cosperpharm

Each batch undergoes:

● Gas chromatography (GC) – purity ≥97.0%

● Non‑aqueous titration – purity ≥97.0%

● Refractive index – confirmatory analysis

● ¹H NMR – structural verification

● Appearance – colorless to light yellow to light orange clear liquid

A comprehensive COA, MSDS (with full GHS information), and certificate of origin accompany every shipment.

Contact Us

Developing antisense oligonucleotides or investigating RNAi mechanisms? Whatever your research with 2′-OMe-Bz-C Phosphoramidite entails, Cosperpharm is here to supply the high‑quality material you need — along with the technical support and reliable delivery your timeline demands. Contact us for a quote, lead time information, or to discuss your specific application requirements.