|
Product Name |
|
|
3'-DMT-2'-F-dA(Bz)-CE Reverse Phosphoramidite |
|
|
3'-DMT-2'-F-dG(iBu)-CE Reverse Phosphoramidite |
|
|
3'-DMT-2'-F-dC(Ac)-CE Reverse Phosphoramidite |
|
|
3'-DMT-2'-F-dU-CE Reverse Phosphoramidite |
|
The 2'-Fluoro modification is highly valued in nucleic acid chemistry for its ability to significantly increase the thermal stability of duplexes and provide exceptional resistance to nuclease degradation. When combined with a reverse phosphoramidite (3'-DMT) chemistry, researchers can introduce inverted 3'-3' linkages at the ends of oligonucleotide sequences. This reverse orientation acts as a robust "cap," blocking exonuclease activity from the termini. Cosperpharma’s 2'-F-Reverse Phosphoramidites are synthesized under strict anhydrous conditions to ensure high coupling efficiency, allowing for the precise construction of these complex, highly stabilized therapeutic candidates.
Synergistic Exonuclease Protection: The combination of the 2'-F sugar modification and the inverted terminal linkage provides a formidable barrier against exonucleases. This dual-action stabilization is critical for extending the serum half-life of oligonucleotide drugs, ensuring they remain intact long enough to reach their target tissues.
Enhanced Binding Affinity: The 2'-F modification locks the sugar into a C3'-endo conformation, which pre-organizes the oligonucleotide for optimal binding to complementary RNA or DNA targets. This leads to higher melting temperatures (Tm) and improved potency in antisense and RNAi applications.
High Purity for Complex Syntheses: Synthesizing oligonucleotides with reverse linkages requires high-fidelity monomers. Every lot of our 2'-F-Reverse Phosphoramidites undergoes rigorous HPLC purification and mass spectrometry confirmation to guarantee the highest purity and reproducible coupling yields.
Comprehensive Regulatory Support: We provide detailed Certificates of Analysis (COA) and Safety Data Sheets (SDS) with every batch. For your drug development needs, customized documentation packages are available to support IND and NDA filings.
We are dedicated to helping you overcome the stability challenges in your oligonucleotide development. If you are interested in exploring the benefits of our 2'-F-Reverse Phosphoramidites for your project, our expert team is ready to assist. Contact Cosperpharma today to request a sample, discuss custom synthesis requirements, or receive a detailed quotation tailored to your research or production scale.
