Boc-Leu-OH·H₂O is a foundational building block in Boc-strategy solid-phase peptide synthesis, serving as the standard reagent for introducing L-leucine residues into synthetic peptides and peptide-based therapeutics. The hydrophobic leucine side chain imparted by Boc-Leu-OH·H₂O is essential for stabilizing protein-protein interactions, membrane-spanning domains, and hydrophobic cores in folded peptides, making this building block indispensable for synthesizing bioactive peptides and peptide drug candidates. In research applications, Boc-Leu-OH·H₂O is widely employed in the construction of combinatorial peptide libraries for structure-activity relationship studies, the synthesis of peptide models for biophysical characterization, and the development of bioactive compounds including cytotoxins and antimicrobial peptides. The monohydrate crystalline form of Boc-Leu-OH·H₂O offers practical advantages over the anhydrous form, including improved crystallinity, consistent stoichiometry, and enhanced stability during long-term storage. The high purity (≥98% TLC) and well-characterized optical activity ([α]²⁰/D = −25±2°, c=2 in AcOH) of Boc-Leu-OH·H₂O ensure reproducible coupling efficiency and consistent peptide quality across synthesis campaigns.
Product Parameters
|
Parameter |
Specification |
|
Product Name |
Boc-Leu-OH·H₂O |
|
CAS Number |
200936-87-4 |
|
Molecular Formula |
|
|
Molecular Weight |
|
|
Appearance |
White to off-white powder or crystals |
|
Melting Point |
85–87 °C |
|
Storage Condition |
Store below +30°C |
Why Cosperpharm?
Purity that ensures coupling success. In Boc-strategy SPPS, the quality of each amino acid building block directly impacts coupling efficiency and final peptide purity. Impurities in Boc-Leu-OH·H₂O — whether residual solvents, deprotected leucine, or stereoisomeric contamination — can lead to failed couplings, deletion sequences, or difficult purifications. Cosperpharm releases every batch with HPLC purity verification (≥98% TLC; ≥99% HPLC), optical rotation confirmation ([α]²⁰/D = −25±2°), and water content analysis to ensure consistent monohydrate stoichiometry.
Documentation that supports your research. Every shipment of Boc-Leu-OH·H₂O includes a Certificate of Analysis (COA) with batch-specific purity data, optical rotation, water content, and identity confirmation. For customers engaged in peptide therapeutic development, Cosperpharm provides DMF-ready documentation, stability summaries, and custom quality agreements upon request.
Flexible supply across all scales. Cosperpharm supplies Boc-Leu-OH·H₂O across the full range of applications — from gram-scale quantities for research and method development, to kilogram-scale batches for process validation and commercial peptide manufacturing. R&D samples ship within 5–7 business days; bulk orders ship within 2–3 weeks.
Technical support from peptide chemistry experts. Our process chemistry team can advise on coupling conditions, deprotection protocols (TFA for Boc removal), and analytical methods for detecting process-related impurities — because we have extensive experience with Boc-protected amino acids across multiple peptide manufacturing campaigns.
Global reach with transparent pricing. Cosperpharm ships to research institutions, CROs, and pharmaceutical manufacturers worldwide, with full customs documentation included. Volume discounts apply at larger scales, and we communicate lead times upfront — no hidden fees, no unexpected delays.
FAQ
A: Boc-Leu-OH·H₂O (N-(tert-Butoxycarbonyl)-L-leucine monohydrate, CAS 200936-87-4) is the monohydrate crystalline form of Boc-protected L-leucine, a standard building block for introducing leucine residues in Boc-strategy solid-phase peptide synthesis.
Q2: What is the difference between Boc-Leu-OH·H₂O and Boc-Leu-OH?
A: Boc-Leu-OH·H₂O is the monohydrate form containing one molecule of water of crystallization per molecule of Boc-Leu-OH. The monohydrate form offers improved crystallinity, consistent stoichiometry, and enhanced stability during storage. The anhydrous form (Boc-Leu-OH) has CAS 13139-15-6 and does not contain water of crystallization.
Application Scenarios
Boc-Leu-OH·H₂O is a standard building block for introducing L-leucine residues in Boc-strategy SPPS. The hydrophobic leucine side chain is essential for stabilizing protein tertiary structures and membrane-spanning domains.
Combinatorial Peptide Library Construction
Used in the synthesis of combinatorial peptide libraries for high-throughput screening and structure-activity relationship (SAR) studies, where leucine contributes to hydrophobic interactions and membrane affinity.
Bioactive Peptide Synthesis
Employed in the synthesis of peptide-based therapeutics, cytotoxins, and antimicrobial peptides where leucine incorporation is critical for biological activity and membrane permeability.
Peptide Model Systems for Biophysical Studies
Used to synthesize peptide models for studying protein folding, hydrophobic interactions, and membrane association in biophysical and structural biology research.
Protein Engineering
Aids in the design of novel peptides and proteins with tailored properties through controlled leucine incorporation.
Process Development & Scale-Up
Pharmaceutical process chemists use Boc-Leu-OH·H₂O for reaction optimization, impurity mapping, and development of scalable manufacturing routes for peptide therapeutics.
Analytical Method Development
Used as a reference standard in developing and validating HPLC methods for peptide synthesis monitoring and quality control of peptide drug substances.
Contact Us
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No. 2 Yangguang 3rd Road, Duodao Chemical Cycle Industrial Park, Jingmen City, Hubei Province, China
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