2'-F-Ac-dCPhosphoramidite

2'-F-Ac-dC Phosphoramidite is a pivotal monomer for the solid-phase synthesis of chemically modified oligonucleotides used in next-generation therapeutics. The 2'-fluoro group it introduces is a cornerstone of modern nucleic acid drug design, providing superior metabolic stability and target affinity compared to natural or 2'-OMe-modified counterparts. 2'-F-Ac-dC Phosphoramidite is a critical component in the manufacture of many FDA-approved antisense drugs and is extensively used in the synthesis of siRNA for RNA interference (RNAi) applications. In research settings, 2'-F-Ac-dC Phosphoramidite enables the creation of highly specific probes for molecular diagnostics, while for process development, 2'-F-Ac-dC Phosphoramidite serves as a reliable standard for optimizing solid-phase coupling efficiencies in synthesizers.

Product Parameters

Quality Assurance at Cosperpharm

Each batch undergoes:

● Gas chromatography (GC) – purity ≥97.0%

● Non‑aqueous titration – purity ≥97.0%

● Refractive index – confirmatory analysis

● ¹H NMR – structural verification

● Appearance – colorless to light yellow to light orange clear liquid

A comprehensive COA, MSDS (with full GHS information), and certificate of origin accompany every shipment.

Product Advantages

1. Exceptional Nuclease Resistance and In Vivo Stability

2'-F-Ac-dC Phosphoramidite incorporates the 2'-fluoro modification, which is widely documented to confer high resistance to nuclease degradation. This stability is crucial for antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs), ensuring an extended pharmacokinetic profile in biological systems.

2. Enhanced Binding Affinity

The 2'-fluoro group pre-organizes the sugar ring into a C3'-endo (RNA-like) conformation, leading to a significant increase in the melting temperature (Tm) and binding affinity for complementary RNA targets.

3. Versatile Building Block for Drug Discovery

Used as a standard monomer in automated synthesizers, 2'-F-Ac-dC Phosphoramidite is compatible with standard solid-phase synthesis chemistry, allowing for seamless integration into existing oligonucleotide production workflows and enabling the creation of chimeric constructs.

4. Dual Role: Therapeutic Component and Diagnostic Probe

2'-F-Ac-dC Phosphoramidite is a cornerstone in both the manufacturing of FDA-approved nucleic acid drugs and the development of highly sensitive and specific molecular diagnostic probes.

FAQ

Q1: What is the role of the 2' Fluoro modification?

A: The 2'-fluoro modification is a chemical alteration to the ribose sugar. It enhances the oligonucleotide's resistance to enzymatic degradation by nucleases and increases its binding affinity (Tm) to a complementary RNA target, making it a key tool for RNA therapeutics.

Q2: What is the difference between 2'-F-Ac-dC and standard DNA phosphoramidites?

A: Unlike a standard DNA amidite which has a hydrogen at the 2' position, 2'-F-Ac-dC has a fluorine atom. This single-atom change significantly increases the stability and binding properties of the resulting oligonucleotide.

Contact us

Need a reliable supply of 2'-F-Ac-dC Phosphoramidite for your RNA therapeutic program? Cosperpharm delivers high-purity monomers with full analytical support. Email us or visit our website to request a quote.