AA3-DLin

AA3-DLin is a next-generation ionizable lipid designed specifically for the formulation of lipid nanoparticles that deliver messenger RNA and other nucleic acid therapeutics. In LNP constructs, AA3-DLin remains neutral at physiological pH, minimizing off-target interactions with blood components, but becomes protonated in the acidic lumen of endosomes, triggering electrostatic destabilization of the endosomal membrane and the release of the nucleic acid cargo into the cytoplasm. Because AA3-DLin contains strategically placed ester bonds, it is efficiently hydrolyzed by endogenous esterases, resulting in rapid metabolic breakdown and significantly reduced accumulation in tissues compared to non-biodegradable ionizable lipids. Researchers working on prophylactic mRNA vaccines, therapeutic cancer vaccines, and CRISPR-Cas9 genome-editing delivery consistently select AA3-DLin as a core component to improve both the potency and the tolerability of their LNP formulations.

Product Parameters

Why Cosperpharm?

Cosperpharm provides AA3-DLin at a purity and batch-to-batch consistency that matches the stringent demands of nucleic acid nanomedicine — because LNP potency begins with lipid quality. Each shipment is supported by an exhaustive Certificate of Analysis detailing HPLC purity, identity confirmation by NMR and mass spectrometry, residual solvent levels, and endotoxin content. We supply the same high-quality AA3-DLin to early-stage discovery labs, preclinical toxicology programs, and pilot GMP manufacturing campaigns, so your screening data reliably predict in vivo performance. Deep-cold logistics, moisture-proof packaging, and full customs documentation come standard, ensuring that AA3-DLin arrives in your facility exactly as it left our quality-controlled, temperature-mapped storage.

Product Advantages

1. Biodegradable ester architecture reduces chronic toxicity

The ester linkages engineered into AA3-DLin are cleavable by intracellular and plasma esterases. Once the lipid fulfills its delivery function, these bonds ensure rapid metabolic clearance from liver and spleen, dramatically reducing the risk of progressive phospholipidosis and prolonged immune activation — a decisive advantage for repeat-dosing regimens.

2. Unbranched linoleyl tails enhance endosomal escape

The two cis-configured double bonds in each linoleyl chain produce a kinked, fluid-phase lipid geometry that favors the formation of non-bilayer structures at endosomal pH. This structural trait accelerates fusion with the endosomal membrane, increasing the cytoplasmic concentration of mRNA and thereby improving transfection efficiency without requiring higher lipid doses.

3. Tertiary amine with optimal pKa for LNP delivery

The amine headgroup of AA3-DLin exhibits an apparent pKa in the range of 6.0–6.5, ensuring that the lipid is minimally charged during circulation but becomes strongly cationic inside acidifying endosomes. This narrow window of protonation is a critical determinant of both hepatic targeting and membrane-destabilizing activity.

4. Ready-to-formulate liquid with excellent solubility

AA3-DLin is supplied as a free-flowing oil that dissolves readily in ethanol and other organic solvents, enabling seamless microfluidic mixing with PEG-lipid, cholesterol, and DSPC to produce uniform LNPs with target particle sizes below 100 nm and polydispersity indices routinely below 0.15.

Storage Conditions

Store AA3-DLin in a tightly sealed, light-resistant container under an argon or nitrogen blanket at –20 °C. Allow the container to equilibrate to ambient temperature before opening to prevent moisture condensation. Avoid repeated freeze-thaw cycles and prolonged exposure to air. Under these conditions, shelf life is validated to 2 years. For GMP programs, long-term and accelerated stability data can be provided to support the Chemistry, Manufacturing, and Controls (CMC) section of regulatory filings.

Contact us

Whether you need 100 milligrams of AA3-DLin for a pilot encapsulation screen or multi-gram batches to advance a lead candidate toward the clinic, Cosperpharm has the lipid quality and application knowledge to accelerate your program. Reach out today and let us handle the chemistry — so you can focus on the biology.