Ac-dC Phosphoramidite

Ac-dC Phosphoramidite is a high‑purity reagent used in the solid‑phase synthesis of DNA oligonucleotides. As a modified phosphoramidite monomer, Ac-dC Phosphoramidite may be utilized in the preparation and synthesis of oligonucleotides. The N⁴‑acetyl protecting group in Ac-dC Phosphoramidite offers distinct advantages over traditional N⁴‑benzoyl protection: the acetyl group is more readily removed under standard deprotection conditions, enabling UltraMild oligonucleotide synthesis protocols. For quality control applications, Ac-dC Phosphoramidite is recognized as DMT‑dC(ac) Phosphoramidite, and the compound is also referred to as 5′‑O‑(4,4′‑dimethoxytrityl)‑N⁴‑acetyl‑2′‑deoxycytidine 3′‑[(2‑cyanoethyl)-(N,N‑diisopropyl)]‑phosphoramidite.

Product Parameters

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Storage Conditions

Store Ac-dC Phosphoramidite in a tightly sealed container under inert atmosphere (nitrogen or argon) at –20 °C for long‑term storage, or at 2–8 °C for short‑term storage. Protect from light and keep the container dry. The compound is moisture‑sensitive; therefore, desiccated storage is strongly recommended. Allow the sealed container to reach ambient temperature before opening to minimize moisture condensation.

Shelf life: 12 months when stored at –20 °C in a sealed container under inert atmosphere.

Handling precautions: Use only in a fume hood. Wear chemical‑resistant gloves, safety goggles, and a lab coat. Avoid generating dust or aerosols. Do not eat, drink, or smoke in work areas. After handling, wash hands thoroughly. Refer to the Safety Data Sheet (SDS) for complete safety information.

Application Scenarios

1. UltraMild DNA Oligonucleotide Synthesis

Ac-dC Phosphoramidite is a high‑purity reagent for the solid‑phase synthesis of DNA oligonucleotides, particularly in UltraMild protocols where rapid deprotection and compatibility with sensitive modifications are required.

2. Modified Oligonucleotide Preparation

As a modified phosphoramidite monomer, Ac-dC Phosphoramidite may be utilized in the preparation and synthesis of oligonucleotides for molecular biology, diagnostics, and therapeutic applications.

3. Fluorescently Labeled DNA Probes

The N⁴‑acetyl protecting group enables milder deprotection conditions, preserving the integrity of sensitive fluorescent dyes and other modifications commonly used in DNA probe synthesis.

4. Antisense Oligonucleotide (ASO) Development

Ac-dC Phosphoramidite serves as a building block for the synthesis of antisense oligonucleotides, where purity and deprotection efficiency are critical for biological activity.

5. siRNA and RNAi Research

While primarily a DNA building block, the acetyl protection strategy is also applicable to RNA synthesis in contexts where RNA base‑labile protecting groups are required.

6. Diagnostic Probe Synthesis

Ac-dC Phosphoramidite is used in the synthesis of diagnostic DNA probes for PCR, in situ hybridization, and other molecular diagnostic applications requiring high‑quality oligonucleotides.

Contact Us

Exploring UltraMild oligonucleotide synthesis or developing modified DNA probes? Whatever your work with Ac-dC Phosphoramidite entails, Cosperpharm is here to supply the high‑quality material you need — along with the technical support and reliable delivery your timeline demands. Contact us for a quote, lead time information, or to discuss your specific application requirements.