Aeea-Aeea

Aeea-Aeea combines a primary amine (nucleophilic, easily converted to maleimide or NHS ester) and a carboxylic acid (activatable for amide coupling), allowing orthogonal, stepwise assembly without cross‑reactivity. The five ether oxygens of Aeea-Aeea confer excellent water solubility and reduce non‑specific protein adsorption. Moreover, the internal secondary amide of Aeea-Aeea adds moderate rigidity while remaining stable under physiological conditions. Whether you need to attach a payload to an antibody or connect an E3 ligase ligand to a target protein binder, Aeea-Aeea delivers reproducibility and high conjugation efficiency.

Cosperpharm supplies this product with ≥97% purity by HPLC, meeting strict quality criteria (residual solvents, water content, titration). We support scales from grams to tens of kilograms, with lead times of 10–20 days.

Product Parameters

Frequently Asked Questions (FAQ)

Q1: What is the exact structure of this compound?

A: H₂N‑CH₂CH₂‑O‑CH₂CH₂‑O‑CH₂CH₂‑O‑CH₂‑CO‑NH‑CH₂CH₂‑O‑CH₂CH₂‑O‑CH₂‑COOH. It contains five ethylene glycol units (three on the amine side, two on the acid side) and one internal amide bond. The molecular weight is 308.33 g/mol, matching the formula C₁₂H₂₄N₂O₇.

Q2: Why is the melting point reported as 133–135 °C?

A: This sharp melting range indicates high crystallinity and purity. Lower purity or polydisperse PEG analogs often melt over a broad range or are waxy solids.

Q3: How should I activate the carboxylic acid?

A: Standard amide coupling: dissolve 1 eq of linker in dry DMF, add 1.1 eq HATU (or EDC) and 2 eq DIPEA, stir for 10 min at 0 °C, then add 1 eq of amine‑containing payload. For aqueous conditions, use EDC/sulfo‑NHS at pH 5.5–6.0.

Handling & Storage

Storage: –20 °C, in a freezer, under inert atmosphere (argon or nitrogen). Keep container tightly sealed and desiccated.

Stability: At least 24 months when stored as recommended. Avoid repeated freeze‑thaw cycles.

Solubility: The compound is soluble in DMSO (use slight heating and sonication if needed) and methanol (slight). For aqueous solutions, consider dissolving first in a small amount of DMSO then diluting with buffer (final DMSO ≤10%). The predicted logP is low, indicating moderate water solubility.

Handling: Allow the vial to warm to room temperature before opening to prevent moisture condensation.

Ordering Information

Ready to incorporate CAS 1143516-05-5 into your next ADC, PROTAC, or bioconjugation project? Request a sample or a quote from Cosperpharm today. We are your trusted supplier for highpurity PEG linkers and specialty intermediates.