Boc-Gly-Gly-OH

Boc-Gly-Gly-OH is a strategically protected dipeptide building block widely recognized for its role in enhancing the efficiency of solid-phase peptide synthesis (SPPS). The Boc group in Boc-Gly-Gly-OH prevents side reactions during peptide assembly, particularly in solid-phase peptide synthesis, while enabling controlled deprotection under standard acidic conditions. As a glycine derivative, Boc-Gly-Gly-OH is valuable for incorporating glycine-glycine sequences into peptides while maintaining control over the deprotection process. Beyond classical peptide synthesis, Boc-Gly-Gly-OH has found application in bioconjugation techniques, where it facilitates the attachment of biomolecules to surfaces or other molecules, supporting the development of targeted drug delivery systems. The pre-formed dipeptide structure of Boc-Gly-Gly-OH makes it an essential reagent in biochemical research and pharmaceutical development.

Product Parameters

Product Advantages

Boc-Gly-Gly-OH is a protected dipeptide that streamlines peptide synthesis by providing a pre-formed glycine-glycine unit. This eliminates the need for sequential glycine coupling steps, reducing synthesis time, minimizing deletion sequence formation, and improving overall peptide yield.

 Robust Boc Protection Strategy

The tert-butyloxycarbonyl (Boc) group in Boc-Gly-Gly-OH provides reliable protection of the N-terminal amino functionality throughout peptide chain assembly. The Boc group prevents side reactions during synthesis and is selectively removed under mild acidic conditions (typically TFA), enabling orthogonal deprotection strategies.

 Versatile Applications Beyond Peptide Synthesis

Beyond classical peptide synthesis, Boc-Gly-Gly-OH is used in bioconjugation techniques, where it facilitates the attachment of biomolecules to surfaces or other molecules, supporting the development of targeted drug delivery systems.

 High Purity for Reproducible Results

Supplied at ≥98% purity (HPLC) with batch-specific characterization, Boc-Gly-Gly-OH ensures reproducible results across synthetic campaigns.

 Essential Reagent for Biochemical Research

Boc-Gly-Gly-OH is widely recognized as an essential reagent in biochemical research and pharmaceutical development, particularly for incorporating glycine residues into peptides while maintaining control over the deprotection process.

Synthetic Route

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Under stirring conditions, amino acids or peptides (1 mmol) were added dropwise to an ethanol solution (1 mL) containing guanidine hydrochloride (15 mmol) and diisobutyl carbonate (2.5–3 mmol), with the reaction temperature maintained at 35–40 °C. The mixture was continuously stirred until a clear solution formed. The ethanol was then evaporated under reduced pressure. The residue was successively washed with distilled water (2 mL) followed by hexane or petroleum ether (2 mL) to yield high-purity N-Boc-amino acids or N-Boc-peptides. For further purification of the product, recrystallization may be performed on the crude product.

Product Quality Assurance

Thorough analytical characterization. Each batch undergoes multi-technique analytical release testing: HPLC purity (≥98%, with high-purity grades up to 99.5% available), melting point verification (132 °C), and appearance inspection (white powder).

Full batch traceability. From raw material procurement through Boc protection, purification, and final packaging, every step is fully documented and traceable. Each batch receives a unique lot number with retention samples maintained in accordance with Cosperpharm quality procedures.

Documentation ready for research and regulatory submission. Every shipment includes a Certificate of Analysis (COA) with batch-specific purity and characterization data, a Material Safety Data Sheet (SDS), and customs documentation. Custom quality agreements and additional documentation packages are available upon request.

Customer-initiated quality audits. Qualified customers are welcome to audit Cosperpharm‘s manufacturing, quality control, and documentation facilities. Please contact our regulatory team to schedule an audit.

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