DOTAP(chloride salt)

DOTAP (chloride salt) is a widely established cationic lipid for the liposomal transfection of DNA (plasmids, bacmids), RNA (mRNA, siRNA), and modified nucleic acids (antisense oligonucleotides) into eukaryotic cells, often without the need for auxiliary lipids. The cationic liposomes formed by DOTAP (chloride salt) encapsulate negatively charged genetic material through electrostatic interactions, forming lipoplexes that fuse with cell membranes and deliver their payload directly into the cytoplasm. DOTAP (chloride salt) demonstrates high transfection efficiency across diverse cell lines including CHO, HEK293T, HeLa, PC3 (prostate cancer), and A549 (lung cancer) cells, with cell viability generally exceeding 80% when optimized protocols are followed. Beyond conventional transfection, DOTAP (chloride salt) has been successfully formulated into lipid nanoparticles (LNPs) and cationic bicelles for siRNA delivery across the blood–brain barrier (BBB), enabling neurotherapeutic applications in Alzheimer‘s disease and other CNS disorders. The predictable performance, ease of formulation, and proven ability of DOTAP (chloride salt) to facilitate intracellular delivery have made it a top‑choice cationic lipid in the field of gene delivery for over three decades. Additionally, DOTAP (chloride salt) can be used for transfection of yeast artificial chromosomes (YACs) with or without serum, further extending its utility in genetic engineering applications.

Product Parameters

Why Cosperpharm? – Our Competitive Advantages

Product Advantages

1. Gold‑Standard Cationic Lipid for Nucleic Acid Transfection

DOTAP (chloride salt) is the most widely established cationic lipid for DNA, RNA, and oligonucleotide transfection. The permanently charged quaternary ammonium headgroup forms stable electrostatic complexes with negatively charged nucleic acids, producing lipoplexes that efficiently enter cells.

2. High Transfection Efficiency with Low Cytotoxicity

DOTAP (chloride salt) demonstrates superior transfection capabilities in PC3 and A549 cell lines compared to commercial reagents, with cell viability generally exceeding 80%. The two unsaturated oleoyl chains confer membrane fluidity that facilitates endosomal escape while minimizing cytotoxic effects.

3. Supports Endosomal Escape — A Key Step in RNA Delivery

The unsaturated oleoyl chains (C18:1, Δ9 cis) promote the lamellar‑to‑inverted hexagonal (H_II) phase transition upon acidification within endosomes, enabling the flip‑flop mechanism that releases nucleic acids into the cytoplasm before lysosomal degradation.

4. Enables siRNA Delivery Across the Blood–Brain Barrier

When formulated into cationic bicelles with DPPC and DHPC, DOTAP (chloride salt) has been successfully used to deliver siRNA across the BBB in a human in vitro model, opening new therapeutic avenues for CNS disorders.

5. Versatile Formulations: Liposomes, LNPs, and Cationic Bicelles

DOTAP (chloride salt) is highly effective across liposomes, LNPs, and cationic bicelles. Its predictable performance makes it a top choice for RNA vaccines, gene therapy products, and neurotherapeutics.

6. Broad Nucleic Acid Compatibility

Efficiently transfects plasmid DNA, bacmids, mRNA, siRNA, antisense oligonucleotides, and YACs — both in serum‑free and serum‑containing media.

FAQ

Q1: What are the primary applications of DOTAP (chloride salt)?

A: Liposomal transfection of DNA, RNA, oligonucleotides, and YACs into eukaryotic cells. Also formulated into LNPs and cationic bicelles for RNA delivery across the BBB and for gene therapy.

Q2: Is DOTAP (chloride salt) hazardous?

A: Some suppliers classify it as not hazardous; others indicate it is a moderate to severe irritant to skin and eyes, and very toxic to aquatic life. Signal Word: Warning. Use in a fume hood with PPE. Prevent release to the environment.

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Developing an LNP‑based RNA therapeutic, generating stable cell lines, or optimizing your transfection workflow? Cosperpharm supplies DOTAP (chloride salt) with the purity, documentation, and supply flexibility that gene delivery researchers and biopharmaceutical manufacturers demand. From research‑grade samples to GMP‑grade bulk quantities, we are your partner for cationic lipid supply.