2-Methyl-L-proline Methyl ester hydrochloride

2-Methyl-L-proline Methyl ester hydrochloride is an essential chiral intermediate in the synthesis of several approved antiviral and anticancer therapeutics, most notably the HCV NS3/4A protease inhibitors glecaprevir and voxilaprevir, where it forms part of the macrocyclic P2 proline cap. The quaternary α‑carbon of 2-Methyl-L-proline Methyl ester hydrochloride prevents enzymatic hydrolysis of the adjacent amide bond, significantly extending the half‑life of peptide‑based drugs. After coupling, the methyl ester of 2-Methyl-L-proline Methyl ester hydrochloride is hydrolyzed to the free acid or directly used in macrocyclization. Cosperpharm verifies the enantiomeric purity of every batch of 2-Methyl-L-proline Methyl ester hydrochloride by chiral HPLC to guarantee >99% e.e., as even minor contamination with the (R)‑enantiomer can compromise the crystallinity and biological activity of the final API.

Product Parameters

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Application Scenarios

1.HCV Protease Inhibitor Synthesis

Key P2 fragment in glecaprevir, voxilaprevir, and earlier boceprevir‑like molecules.

2.SARS‑CoV‑2 Protease

Inhibitor R&D Incorporated into peptidomimetic inhibitors targeting the 3CLpro enzyme.

3.Anticancer Peptide Conjugates

Used to stabilize the turn conformation of cyclic peptides targeting MDM2/p53 or integrins.

4.Macrocyclic Drug Discovery

Serves as a rigid proline surrogate in the design of macrocyclic peptides and depsipeptides.

5.Chemical Ligation Studies

The quaternary α‑center is used to study the effect of conformational pre‑organization on ligation rates.

Contact Us

To incorporate this constrained proline building block into your next peptidomimetic lead, contact Cosperpharm’s chiral amino acid specialist — we can provide a quote, sample, and discuss custom α‑alkylproline derivatives tailored to your sequence.