2'-OMe-U Phosphoramidite

2'-OMe-U Phosphoramidite is a second-generation, high-purity RNA monomer designed to enhance the drug-like properties of oligonucleotide therapeutics. The incorporation of the 2'-O-methyl group via 2'-OMe-U Phosphoramidite is a widely adopted strategy to improve the stability and reduce the immunogenicity of small interfering RNAs (siRNAs) and antisense oligonucleotides (ASOs). 2'-OMe-U Phosphoramidite is essential for researchers developing RNA-based drugs, as it balances high target affinity with a superior safety profile. Furthermore, 2'-OMe-U Phosphoramidite is fully compatible with standard RNA synthesis platforms, making the production of modified RNA straightforward. For both academic discovery and industrial process development, 2'-OMe-U Phosphoramidite is a reliable and highly effective building block.

Product Parameters

Product Advantages

1. Clinically Validated Modification

The 2'-O-methyl (2'-OMe) modification is a well-established chemical strategy used in many FDA-approved oligonucleotide drugs to improve their therapeutic index.

2. Superior Nuclease Resistance

Oligonucleotides synthesized with 2'-OMe-U Phosphoramidite exhibit dramatically enhanced resistance to degradation by serum nucleases, leading to a longer half-life and better in vivo activity compared to unmodified RNA.

3. Improved Safety Profile

Studies have shown that 2'-OMe modifications can reduce the immunostimulatory potential of RNA molecules, minimizing unwanted off-target effects and inflammation seen with some unmodified RNA sequences.

4. High Target Affinity

Unlike some other modifications, 2'-OMe confers an increased binding affinity (Tm) for its complementary RNA target, making it an ideal choice for potent antisense and siRNA agents.

FAQ

Q1: How does 2'-OMe-U compare to 2'-Fluoro (2'-F) modifications?

A: Both enhance stability, but 2'-OMe is generally less nuclease-resistant than 2'-F. However, 2'-OMe has a better safety profile and is less immunogenic, making it a preferred choice for many in vivo applications.

Q2: Is 2'-OMe-U Phosphoramidite compatible with standard RNA synthesis?

A: Yes, it is a standard RNA phosphoramidite and is fully compatible with all major RNA/DNA synthesizers and standard synthesis cycles.

Product Quality Assurance

Cosperpharm is dedicated to providing high-quality 2'-OMe-U Phosphoramidite for your critical RNA synthesis applications:

● Analytical Rigor: Each batch undergoes strict quality control using HPLC (≥98.0% purity) and ³¹P NMR to ensure high coupling efficiency and batch-to-batch consistency.

● Moisture Control: Karl Fischer (KF) analysis is performed to confirm low water content, a critical parameter for amidite stability and performance.

● Full Batch Traceability: Complete documentation from raw material sourcing through final release, with a unique lot number for each batch.

● Regulatory Support: A detailed Certificate of Analysis (COA) is included with every order. Quality agreements and DMF-ready documentation are available upon request for GMP programs.

Contact us

Scaling up RNA synthesis? Let Cosperpharm provide consistent 2'-OMe-U Phosphoramidite. Contact us for batch-specific COAs and volume pricing.