2'-OMe-U Phosphoramidite is a second-generation, high-purity RNA monomer designed to enhance the drug-like properties of oligonucleotide therapeutics. The incorporation of the 2'-O-methyl group via 2'-OMe-U Phosphoramidite is a widely adopted strategy to improve the stability and reduce the immunogenicity of small interfering RNAs (siRNAs) and antisense oligonucleotides (ASOs). 2'-OMe-U Phosphoramidite is essential for researchers developing RNA-based drugs, as it balances high target affinity with a superior safety profile. Furthermore, 2'-OMe-U Phosphoramidite is fully compatible with standard RNA synthesis platforms, making the production of modified RNA straightforward. For both academic discovery and industrial process development, 2'-OMe-U Phosphoramidite is a reliable and highly effective building block.
|
Parameter |
Specification |
|
Product Name |
2'-OMe-U Phosphoramidite |
|
CAS Number |
110764-79-9 |
|
Molecular Formula |
C₄₀H₄₉N₄O₉P |
|
Molecular Weight |
760.82 g/mol |
|
Purity (HPLC) |
≥98.0% |
|
Physical Form |
Powder |
|
Appearance |
White to off-white powder |
|
Storage Condition |
Sealed in dry,2-8°C |
|
Solubility |
DMSO:100mg/mL(131.44mM) |
The 2'-O-methyl (2'-OMe) modification is a well-established chemical strategy used in many FDA-approved oligonucleotide drugs to improve their therapeutic index.
Oligonucleotides synthesized with 2'-OMe-U Phosphoramidite exhibit dramatically enhanced resistance to degradation by serum nucleases, leading to a longer half-life and better in vivo activity compared to unmodified RNA.
Studies have shown that 2'-OMe modifications can reduce the immunostimulatory potential of RNA molecules, minimizing unwanted off-target effects and inflammation seen with some unmodified RNA sequences.
Unlike some other modifications, 2'-OMe confers an increased binding affinity (Tm) for its complementary RNA target, making it an ideal choice for potent antisense and siRNA agents.
Q1: How does 2'-OMe-U compare to 2'-Fluoro (2'-F) modifications?
A: Both enhance stability, but 2'-OMe is generally less nuclease-resistant than 2'-F. However, 2'-OMe has a better safety profile and is less immunogenic, making it a preferred choice for many in vivo applications.
Q2: Is 2'-OMe-U Phosphoramidite compatible with standard RNA synthesis?
A: Yes, it is a standard RNA phosphoramidite and is fully compatible with all major RNA/DNA synthesizers and standard synthesis cycles.
Cosperpharm is dedicated to providing high-quality 2'-OMe-U Phosphoramidite for your critical RNA synthesis applications:
● Analytical Rigor: Each batch undergoes strict quality control using HPLC (≥98.0% purity) and ³¹P NMR to ensure high coupling efficiency and batch-to-batch consistency.
● Moisture Control: Karl Fischer (KF) analysis is performed to confirm low water content, a critical parameter for amidite stability and performance.
● Full Batch Traceability: Complete documentation from raw material sourcing through final release, with a unique lot number for each batch.
● Regulatory Support: A detailed Certificate of Analysis (COA) is included with every order. Quality agreements and DMF-ready documentation are available upon request for GMP programs.
Scaling up RNA synthesis? Let Cosperpharm provide consistent 2'-OMe-U Phosphoramidite. Contact us for batch-specific COAs and volume pricing.
Address
No. 2 Yangguang 3rd Road, Duodao Chemical Cycle Industrial Park, Jingmen City, Hubei Province, China
Tel
![1-[2,3-Bis[(9Z,12Z)-octadeca-9,12-dien-1-yloxy]propyl]pyrrolidine](https://www.cosperpharma.com/upload/8820/1-2-3-bis-9z-12z-octadeca-9-12-dien-1-yloxy-propyl-pyrrolidine-349384.webp "1-[2,3-Bis[(9Z,12Z)-octadeca-9,12-dien-1-yloxy]propyl]pyrrolidine")
![1,2,4-Triazolo[4,3-a]pyridin-3(2H)-one](https://www.cosperpharma.com/upload/8820/1-2-4-triazolo-4-3-a-pyridin-3-2h-one-440735.webp "1,2,4-Triazolo[4,3-a]pyridin-3(2H)-one")
