3-[3-imidazol-1-ylpropyl-[3-oxo-3-(2-tetradecanoxyethoxy)propyl]amino]propanoic acid 2-tetradecanoxyethyl ester

3-[3-imidazol-1-ylpropyl-[3-oxo-3-(2-tetradecanoxyethoxy)propyl]amino]propanoic acid 2-tetradecanoxyethyl ester is an ionizable lipid designed to expand the toolbox of LNP-enabled nucleic acid therapies, especially where conventional amine-only lipids fail to provide the desired balance of mRNA protection and cytoplasmic release. The imidazole group contributes a secondary protonation equilibrium, and the ether-linked tetradecanol chains enhance chemical stability relative to fully esterified hydrophobic domains. When incorporated into a four-component LNP alongside a PEG-lipid, cholesterol, and a helper phospholipid, 3-[3-imidazol-1-ylpropyl-[3-oxo-3-(2-tetradecanoxyethoxy)propyl]amino]propanoic acid 2-tetradecanoxyethyl ester generates nanoparticles with uniform size distribution, high encapsulation efficiency, and dose-dependent transgene expression. Beyond its primary use in mRNA delivery, 3-[3-imidazol-1-ylpropyl-[3-oxo-3-(2-tetradecanoxyethoxy)propyl]amino]propanoic acid 2-tetradecanoxyethyl ester is also being investigated as a carrier for small interfering RNA, self-amplifying RNA, and DNA payloads, making it a versatile asset across the entire gene-medicine pipeline.

Product Parameters

Why Cosperpharm?

When your LNP screening campaign demands an imidazole-bearing ionizable lipid with tightly controlled purity, Cosperpharm delivers 3-[3-imidazol-1-ylpropyl-[3-oxo-3-(2-tetradecanoxyethoxy)propyl]amino]propanoic acid 2-tetradecanoxyethyl ester alongside the analytical documentation that preclinical advancement requires. We test each lot for identity (HRMS, ¹H NMR), chromatographic purity, residual solvent profile, and endotoxin, so you can move from microfluidic formulation to in vivo efficacy without re-characterizing your key excipient. Our lipids leave Cosperpharm under cold-chain protocols validated for stability, and we offer the same responsive technical support that has made us a trusted supplier to RNA therapeutics developers across North America, Europe, and Asia.

Application Scenarios

1.mRNA Vaccine LNP Formulation

3-[3-imidazol-1-ylpropyl-[3-oxo-3-(2-tetradecanoxyethoxy)propyl]amino]propanoic acid 2-tetradecanoxyethyl ester is used as the ionizable lipid in prophylactic and therapeutic mRNA vaccines to achieve high antigen expression and robust immune stimulation.

2.Self-Amplifying RNA Delivery

Adapted for larger replicon RNA payloads, exploiting the lipid’s balanced membrane-destabilizing properties.

3. siRNA Hepatic Silencing

 LNPs containing this lipid deliver siRNA to hepatocytes for sustained gene knockdown in metabolic and genetic liver disease models.

4. DNA Plasmid Delivery

Serves as a vector for non-viral plasmid DNA delivery, particularly in immuno-oncology applications where transient transgene expression is desired.

5. Combinatorial Lipid Library Screening

Included as a variant in structure-activity relationship panels aimed at deconvoluting the contribution of imidazole protonation to overall LNP efficacy.

6. Process Development & Scale-Up

Cosperpharm supplies multi-gram batches for optimizing microfluidic mixing parameters, tangential flow filtration, and lyophilization cycle development.

Product Quality Assurance

Every lot of 3-[3-imidazol-1-ylpropyl-[3-oxo-3-(2-tetradecanoxyethoxy)propyl]amino]propanoic acid 2-tetradecanoxyethyl ester released by Cosperpharm is authenticated by ¹H NMR and high-resolution mass spectrometry, quantified by HPLC-ELSD (≥95%), and evaluated for residual solvents (headspace GC) and endotoxin (LAL). Retention samples are maintained under controlled conditions for the entire shelf life, enabling retrospective analysis if needed. Our analytical methods are benchmarked against ICH Q2(R1) and can be transferred to your quality control laboratory along with reference standards. A comprehensive technical dossier — suitable for direct inclusion in a Drug Master File — is available to support your regulatory filings.

Contact us

From lead identification to IND-enabling toxicology, the right ionizable lipid can make the difference between an LNP that barely expresses and one that transforms a gene therapy program. Contact Cosperpharm to discuss your custom lipid requirements, request a sample of 3-[3-imidazol-1-ylpropyl-[3-oxo-3-(2-tetradecanoxyethoxy)propyl]amino]propanoic acid 2-tetradecanoxyethyl ester, or secure a dedicated supply agreement for your clinical pipeline.