Boc-His(Trt)-Ala-OH

Boc-His(Trt)-Ala-OH is a pivotal protected dipeptide intermediate widely employed in the pharmaceutical synthesis of GLP-1 receptor agonists, including semaglutide, tirzepatide, and other peptide-based therapeutics for type 2 diabetes and obesity management. The compound features a histidine residue protected at the α-amino position with a Boc group and at the imidazole side chain with a trityl (Trt) group, coupled to an alanine residue with a free C-terminal carboxyl, creating a molecular scaffold that combines the structural complexity of the histidine-alanine sequence with orthogonal protection for selective deprotection during peptide chain assembly. As a key fragment in GLP-1 peptide synthesis, Boc-His(Trt)-Ala-OH enables researchers to incorporate the critical His-Ala sequence at defined positions within the peptide chain, streamlining the synthesis of complex therapeutic peptides. The orthogonal protection strategy of Boc-His(Trt)-Ala-OH allows for selective removal of the Boc group under mild acidic conditions (e.g., TFA in DCM) while the Trt group remains stable, enabling precise control over the peptide assembly process. Furthermore, Boc-His(Trt)-Ala-OH serves as a versatile building block in the synthesis of various bioactive peptides beyond the GLP-1 class, including peptide-based drugs targeting viral infections, cancer, and cardiovascular conditions.

 

Product Parameters

---

Parameter	Specification
Product Name	Boc-His(Trt)-Ala-OH
CAS Number	1454254-70-6
Molecular Formula	C₃₃H₃₆N₄O₅
Molecular Weight	568.66 g/mol
Density	1.19 ± 0.1 g/cm³ (predicted)
Boiling Point	787.6 ± 60.0 °C (predicted)
pKa	3.33±0.10

 

Why Cosperpharm?

---

When your GLP-1 peptide manufacturing campaign demands high-purity protected dipeptide intermediates, Cosperpharm delivers Boc-His(Trt)-Ala-OH with the analytical rigor, documentation depth, and supply flexibility that pharmaceutical peptide manufacturers require.

 

Peptide synthesis precision starts here. The His-Ala sequence in Boc-His(Trt)-Ala-OH is a critical structural element in GLP-1 receptor agonists such as semaglutide and tirzepatide. Any impurity or epimerization in this dipeptide building block will compromise the biological activity and purity of your final peptide product. Our analytical release protocol for Boc-His(Trt)-Ala-OH includes HPLC purity verification (≥99.0%), appearance verification, identity confirmation, and single impurity control (≤0.1%) — because your final peptide bioactivity depends on the quality of this building block.

 

Documentation that supports regulatory submission. Every shipment of Boc-His(Trt)-Ala-OH includes a Certificate of Analysis (COA) with batch-specific HPLC purity, chromatographic traceability, and impurity data — structured for direct insertion into your peptide drug substance documentation package. Custom quality agreements and stability summaries are available upon request.

 

Flexible supply across all development stages. Cosperpharm supplies Boc-His(Trt)-Ala-OH across the full range of applications — from gram-scale R&D quantities for peptide library screening and process development, to kilogram-scale batches for pilot production, to multi-kilogram quantities for commercial GLP-1 peptide manufacturing. R&D samples ship within 5–7 business days; bulk orders ship within 2–3 weeks.

 

Technical support for complex peptide synthesis. Our process chemistry team can advise on the selection of coupling conditions for incorporating Boc-His(Trt)-Ala-OH into GLP-1 peptide sequences, compatibility with various SPPS resins and deprotection strategies, and analytical methods for detecting process-related impurities and epimeric byproducts — because we have worked extensively with this protected dipeptide across multiple GLP-1 manufacturing campaigns.

 

Global reach with transparent pricing. Cosperpharm ships to peptide manufacturers, CROs, and research laboratories worldwide, with full customs documentation included. Volume discounts apply at kilogram scales, and we communicate lead times upfront — no hidden fees, no unexpected delays.

 

Product Advantages

---

 Orthogonally Protected Dipeptide Building Block

Boc-His(Trt)-Ala-OH features a histidine-alanine dipeptide scaffold with three distinct functional elements: a Boc-protected α-amino group for selective deprotection under acidic conditions, a Trt-protected imidazole side chain for robust protection during chain assembly, and a free C-terminal carboxyl for straightforward coupling to subsequent amino acid residues. This orthogonal protection strategy enables precise control over the peptide assembly process.

 

  Critical Fragment for GLP-1 Peptide Synthesis

Boc-His(Trt)-Ala-OH is an essential building block for the synthesis of GLP-1 receptor agonists, including semaglutide, tirzepatide, and other peptide-based therapeutics for type 2 diabetes and obesity. The His-Ala sequence is a conserved motif in the GLP-1 peptide family, making this dipeptide intermediate indispensable for GLP-1 drug development.

 

 Robust Trityl Protection for Histidine

The trityl (Trt) group on Boc-His(Trt)-Ala-OH provides exceptional protection for the histidine imidazole side chain during SPPS, preventing undesired side reactions and racemization. The Trt group is selectively removable under mild acidic conditions (e.g., TFA in DCM with scavengers), enabling clean deprotection without compromising the peptide sequence.

 

 High-Purity Grade for Pharmaceutical Applications

Boc-His(Trt)-Ala-OH is available at ≥99.0% HPLC purity with single impurity控制在 ≤0.1%, meeting the rigorous quality standards required for pharmaceutical peptide manufacturing. Each batch undergoes comprehensive analytical release testing to ensure consistent quality and batch-to-batch reproducibility.

 

 Broad Utility Beyond GLP-1 Peptides

Beyond GLP-1 therapeutics, Boc-His(Trt)-Ala-OH serves as a versatile building block in the synthesis of various bioactive peptides targeting viral infections (hepatitis, HIV, SARS), cancer, and cardiovascular conditions. The protected dipeptide scaffold enables researchers to incorporate the histidine-alanine motif into diverse peptide sequences for drug discovery and development.

 

Storage Conditions

---

Store Boc-His(Trt)-Ala-OH in a tightly sealed container at 2–8 °C (refrigerated) or at −15 °C for long-term storage. Keep the container dry, protected from light, and stored in a clean, well-ventilated area away from strong oxidizing agents, strong acids, and sources of ignition. Protect from moisture; desiccated storage is strongly recommended. Allow the sealed container to reach ambient temperature before opening to minimize moisture condensation.

 

Shelf life: 2 years when stored at 2–8 °C in a sealed container under recommended conditions.

 

Handling precautions: Use only in a fume hood. Wear chemical-resistant gloves, safety goggles, and a lab coat. Avoid generating dust or aerosols. Do not eat, drink, or smoke in work areas. After handling, wash hands thoroughly. Contaminated clothing should be removed and washed before reuse. Refer to the Safety Data Sheet (SDS) for complete safety information.

 

Contact Us

---

Looking for Boc-His(Trt)-Ala-OH to support your GLP-1 peptide development program or commercial manufacturing campaign? Cosperpharm is your trusted partner for high-quality protected dipeptide intermediates. Our team is ready to provide the products, documentation, and technical support you need. Reach out today — we look forward to collaborating with you.