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Product Name |
CAS No. |
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dSpacer Phosphoramidite |
129821-76-7 |
|
|
Reverse abasic Phosphoramidite |
401813-16-9 |
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Chemical phosphorylation reagent I |
108783-02-4 |
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|
Chemical phosphorylation reagent II |
202284-84-2 |
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Tailored Flexibility and Spacing: The dSpacer (deoxyribose) and Reverse abasic phosphoramidites are invaluable for introducing precise gaps or abasic sites into oligonucleotide sequences. The dSpacer creates a flexible, non-nucleosidic linkage, while the Reverse abasic monomer allows for the introduction of an inverted abasic site, often used to block exonuclease degradation or to mimic specific DNA damage lesions in biological studies.
Efficient Terminal Phosphorylation: Our Chemical Phosphorylation Reagents I and II provide a robust, alternative method for introducing a 5'-phosphate group without the need for enzymatic treatment (such as T4 polynucleotide kinase). This chemical approach is highly efficient, cost-effective, and particularly advantageous for large-scale synthesis or when the target sequence is sensitive to enzymatic conditions. The resulting 5'-phosphate is essential for downstream applications like ligation or cloning.
High Purity for Reliable Synthesis: All monomers in this category are synthesized under strictly controlled conditions and undergo rigorous HPLC purification. This ensures high coupling efficiency and minimizes the formation of unwanted side products, guaranteeing that your complex synthetic designs are executed flawlessly.
Comprehensive Documentation: Every batch is accompanied by a detailed Certificate of Analysis (COA) and Safety Data Sheet (SDS), providing the transparency and reliability required for your quality assurance and regulatory needs.
Cosperpharma’s spacer and phosphorylation reagents are essential for a variety of specialized molecular biology and therapeutic applications:
Probe Design: Utilizing dSpacers to optimize the distance between fluorescent dyes and quenchers in molecular beacons, or to bridge mismatched regions in complex hybridization probes.
Nuclease Resistance: Employing Reverse abasic phosphoramidites to cap the ends of therapeutic oligonucleotides, protecting them from exonuclease degradation.
Ligation and Cloning: Using Chemical Phosphorylation Reagents to generate 5'-phosphorylated oligonucleotides ready for T4 DNA ligase-mediated joining in gene assembly.
Structural Biology: Introducing abasic sites to study DNA repair mechanisms and the structural dynamics of nucleic acid-protein interactions.
We are committed to providing you with the comprehensive toolkit necessary for your most complex synthesis challenges. Whether you require our dSpacer monomers or our efficient chemical phosphorylation reagents, our team is ready to support your work. Contact Cosperpharma today to request a detailed specification sheet, inquire about our latest batch data, or receive a customized quotation for your project.
