Fmoc-Phe-OH

Fmoc-Phe-OH serves as the definitive building block for introducing L‑phenylalanine residues into peptide sequences via Fmoc solid‑phase peptide synthesis. As a standard Fmoc‑protected amino acid, Fmoc-Phe-OH features high purity with very low levels of dipeptide, free‑amino acids and acetic acid impurities, making it suitable for both research and process production of peptides. The base‑lability of the Fmoc group ensures rapid and complete deprotection under standard SPPS conditions, allowing efficient peptide chain elongation without compromising the integrity of side‑chain protecting groups. Additionally, Fmoc-Phe-OH finds expanding applications in materials science, including the preparation of amino acid‑based double network hydrogels for photocontrolled drug release, showing promise in drug delivery systems. As a self‑assembling motif, Fmoc-Phe-OH has been extensively investigated for the formation of functional biomaterials applicable in tissue engineering and regenerative medicine.

Product Parameters

Why Cosperpharm?

When your peptide synthesis campaign demands consistent quality and reliable delivery, Cosperpharm delivers Fmoc-Phe-OH with the analytical rigor and supply flexibility that research and pharmaceutical customers expect.

Consistent quality from batch to batch. Our Fmoc-Phe-OH undergoes comprehensive analytical release testing, including HPLC purity (≥99.0%), chiral purity (enantiomeric excess ≥99.8%), water content (Karl Fischer ≤2.0%), residual solvent analysis, and specific optical rotation verification. Each batch is accompanied by a full Certificate of Analysis — because reproducibility in peptide synthesis begins with the quality of your building blocks.

Documentation that supports your research. For customers engaged in cGMP peptide manufacturing or IND‑filing programs, Cosperpharm provides DMF‑ready documentation packages, stability summaries, and custom quality agreements upon request. Our analytical data includes impurity profiling for dipeptide by‑products such as Fmoc‑Phe‑Phe‑OH and Fmoc‑β‑Ala‑OH, ensuring complete traceability.

Flexible supply across all scales. Whether you need milligram quantities for SAR studies, gram quantities for peptide library synthesis, or kilogram quantities for process development and commercial production, Cosperpharm supplies Fmoc-Phe-OH in a range of pack sizes. R&D samples ship within 3–5 business days; bulk orders ship within 2–3 weeks.

Technical support for your synthetic workflow. Our process chemistry team can advise on SPPS coupling conditions, deprotection protocols, and compatibility with various resin types — because we have worked extensively with Fmoc amino acids across multiple peptide manufacturing campaigns. From standard Fmoc SPPS to green chemistry approaches like aqueous‑phase peptide synthesis, we provide the building blocks that enable your success.

Global reach with transparent pricing. Cosperpharm ships to peptide synthesis laboratories, CROs, and pharmaceutical manufacturers worldwide, with full customs documentation included. Volume discounts apply at larger scales, and we communicate lead times upfront — no hidden fees, no unexpected delays.

Synthetic Route

Preparation via Fmoc Protection of L‑Phenylalanine

The industrial synthesis of Fmoc-Phe-OH proceeds through the selective protection of the α‑amino group of L‑phenylalanine with the Fmoc group. A representative method is described as follows:

Step 1 — Dissolution of L‑phenylalanine: Dissolve L‑phenylalanine in an aqueous solution containing a base such as sodium bicarbonate (NaHCO₃) to convert the amino acid into its anionic form, facilitating nucleophilic attack.

Step 2 — Fmoc protection: Add 9‑fluorenylmethyloxycarbonyl chloride (Fmoc‑Cl) to the reaction mixture in the presence of an organic co‑solvent such as dioxane or acetone. The reaction proceeds under controlled temperature conditions to selectively protect the α‑amino group.

Step 3 — Workup and isolation: After completion of the reaction, acidify the mixture to protonate the carboxylic acid group. Extract the product with an organic solvent, wash to remove excess reagents and by‑products, and concentrate.

Step 4 — Precipitation and drying: Precipitate the crude product by adding diethyl ether, followed by centrifugation or filtration. Recrystallization from a suitable solvent system yields pure Fmoc-Phe-OH.

Step 5 — Drying and packaging: Dry the product under vacuum to remove residual solvents. Package under inert atmosphere for long‑term storage.

Product Quality Assurance

Cosperpharm has established a comprehensive quality assurance system for Fmoc-Phe-OH that meets the rigorous expectations of both research peptide synthesis and cGMP pharmaceutical manufacturing:

Thorough analytical characterization. Each batch of Fmoc-Phe-OH undergoes multi‑technique analytical release testing: HPLC purity (≥99.0%), TLC purity (≥98%), enantiomeric purity (≥99.8%), D‑enantiomer content (≤0.3%), water content (Karl Fischer ≤2.0%), specific optical rotation verification, and appearance inspection. Impurity profiling includes quantification of dipeptide impurities such as Fmoc‑Phe‑Phe‑OH (≤0.1%) and Fmoc‑β‑Ala‑OH (≤0.1%).

Full batch traceability with retention samples. From raw material procurement (L‑phenylalanine and Fmoc‑Cl from qualified suppliers) through protection reaction, workup, recrystallization, drying, and final packaging, every step is fully documented and traceable. Each batch receives a unique lot number with sufficient retention samples maintained in accordance with Cosperpharm quality procedures.

Stability monitoring program. Cosperpharm conducts ongoing stability studies under recommended storage conditions: long‑term storage at –20°C under inert atmosphere, with accelerated stability studies (40°C / 75% RH) conducted on representative batches. Stability summaries are updated regularly, with full data packages available to support customer regulatory submissions.

Documentation ready for regulatory submission. Every shipment includes a Certificate of Analysis (COA) with batch‑specific purity and characterization data, a Material Safety Data Sheet (SDS), and customs documentation for international delivery. For customers preparing IND or ANDA filings for peptide APIs, Cosperpharm provides DMF‑ready documentation packages, method validation reports, stability data, and custom quality agreements upon request.

Customer‑initiated quality audits. Qualified customers are welcome to audit Cosperpharm‘s manufacturing, quality control, and documentation facilities. Please contact our regulatory team to schedule an audit.

Contact Us

Ready to secure Fmoc-Phe-OH for your peptide synthesis project? Whether you are developing a novel therapeutic peptide, building a peptide library for drug discovery, or scaling up cGMP production, Cosperpharm offers the quality, documentation, and supply reliability you need.

Reach out to our customer service team for a quote, technical support, or to request custom packaging. We look forward to supporting your next success.