Fmoc-L-4-Nitro-Phe-OH

Fmoc-L-4-Nitro-Phe-OH serves as a critical building block for Fmoc solid-phase peptide synthesis, enabling the incorporation of 4-nitrophenylalanine into synthetic peptides while maintaining the native L-configuration. The nitro group in Fmoc-L-4-Nitro-Phe-OH provides a UV-active chromophore for efficient monitoring of coupling and deprotection steps during SPPS, while also offering a versatile handle for post-synthetic modifications through selective reduction to an aniline. Additionally, Fmoc-L-4-Nitro-Phe-OH is widely used in drug discovery research for the development of peptide-based therapeutics targeting specific biological pathways, and in chemical biology for studying protein interactions and cellular functions. For researchers requiring high-quality L-amino acid building blocks for bioactive peptide synthesis, Fmoc-L-4-Nitro-Phe-OH delivers the purity, consistency, and performance demanded by modern peptide synthesis workflows.

Product Parameters

Why Cosperpharm?

When your bioactive peptide synthesis or drug discovery program demands high-quality L-amino acid building blocks, Cosperpharm delivers Fmoc-L-4-Nitro-Phe-OH with the analytical rigor and supply flexibility that your research requires.

High-purity material for reliable SPPS. Our Fmoc-L-4-Nitro-Phe-OH undergoes rigorous analytical release testing including HPLC purity verification (≥99%), melting point verification (179–181 °C), optical rotation confirmation ([α]²⁰ᴅ = –34 ± 1°, c=1 in DMF), and identity confirmation via 1H NMR and mass spectrometry. This thorough characterization ensures batch-to-batch consistency and reproducibility in your peptide synthesis campaigns.

Documentation that supports your research integrity. Every shipment includes a Certificate of Analysis (COA) with batch-specific purity and characterization data—structured for direct insertion into your laboratory documentation, method validation reports, or regulatory submissions. Custom quality agreements are available upon request.

Flexible supply across all research phases. Cosperpharm supplies Fmoc-L-4-Nitro-Phe-OH from 1g–50g research quantities for early-stage discovery and method development, to 100g–1kg+ scales for process optimization and pilot production. R&D samples ship within 5–7 business days; bulk orders ship within 2–3 weeks.

Technical support for modified peptide synthesis. Our process chemistry team can advise on coupling conditions for 4-nitrophenylalanine derivatives, strategies for post-synthetic reduction of the nitro group to aniline, and analytical methods for monitoring coupling efficiency and peptide purity. Our expertise comes from extensive work with this Fmoc-protected L-phenylalanine derivative.

Global reach with transparent pricing. Cosperpharm ships to pharmaceutical manufacturers, CROs, biotechnology companies, and academic institutions worldwide. Volume discounts apply at kilogram scales, and we communicate lead times upfront—no hidden fees, no unexpected delays.

Synthetic Route

Suspend 37.7 g (0.179 mol) of (S)-2-amino-3-(4-nitophenyl)propionic acid in 200 mL of 2% NaOH aqueous solution, then add 80 mL of acetonitrile until complete dissolution. Add a suspension of 61.8 g (0.183 mol) of 9-fluoromethyl-N-succinimide carbamate in 100 mL of acetonitrile to the solution. Stir the reaction mixture at room temperature for 3 hours, then acidify with 5% HCl to pH 4–5. Filter to collect the white precipitate, wash with ethyl acetate, and dry. Recrystallize the product using an ethanol-dioxane (50:50) mixture.

Quality Assurance at Cosperpharm

Each batch undergoes:

 Gas chromatography (GC) – purity ≥97.0%

 Non‑aqueous titration – purity ≥97.0%

 Refractive index – confirmatory analysis

 ¹H NMR – structural verification

 Appearance – colorless to light yellow to light orange clear liquid

A comprehensive COA, MSDS (with full GHS information), and certificate of origin accompany every shipment.

Contact Us

Looking for Fmoc-L-4-Nitro-Phe-OH of uncompromising quality for your peptide synthesis? Reach out to Cosperpharm—we‘re ready to provide quotes, share technical data sheets, and support your custom sourcing requirements.