Fmoc-Thr-OH.H2O

Fmoc-Thr-OH·H₂O is an essential building block in Fmoc‑based solid‑phase peptide synthesis, serving as the protected precursor for incorporating L‑threonine into synthetic peptides with complete stereochemical integrity. In peptide synthesis workflows, Fmoc-Thr-OH·H₂O is coupled to resin-bound peptide chains using standard coupling reagents such as HBTU/HOBt, HATU, or PyBOP in the presence of a tertiary amine base like DIPEA. The free side‑chain hydroxyl group of Fmoc-Thr-OH·H₂O remains unblocked during chain assembly, which is particularly advantageous for constructing peptides where the threonine side chain participates in hydrogen bonding networks or serves as a site for subsequent functionalization. Fmoc-Thr-OH·H₂O has been successfully employed in the synthesis of glycopeptides, phosphopeptides for cellular signaling studies, and a wide range of therapeutic peptides [2†L5-L8]. The monohydrate crystalline form of Fmoc-Thr-OH·H₂O offers superior handling properties and consistent performance in automated peptide synthesizers. Fmoc-Thr-OH·H₂O is available at ≥98% HPLC purity from Cosperpharm, with full analytical characterization and batch traceability to support both research‑scale discovery and GMP‑grade manufacturing campaigns.

Product Parameters

Why Cosperpharm？

Cosperpharm has established a strong reputation as a trusted supplier of high‑purity Fmoc‑protected amino acids and peptide building blocks for the global pharmaceutical and biotechnology industries. We understand that the success of your peptide synthesis campaigns — whether at small‑scale discovery, pilot production, or full GMP manufacturing — depends on consistent raw material quality, rigorous analytical traceability, and responsive technical support from your suppliers.

Quality is built into every batch. Every shipment of Fmoc-Thr-OH·H₂O from Cosperpharm undergoes comprehensive analytical release testing: HPLC purity verification (≥98.0% with full chromatographic traceability), appearance verification (white to off‑white crystalline powder), water content determination by Karl Fischer, identity confirmation by ¹H NMR, and specific optical rotation measurement to confirm stereochemical integrity. We maintain in‑process controls from raw material sourcing through synthesis, purification, drying, and final packaging — so you can be confident that every gram meets your exact specifications.

Documentation that supports your regulatory submissions. For customers working on GMP peptide manufacturing or ANDA filings involving peptide drug substances, Cosperpharm provides DMF‑ready documentation packages, method validation reports, and comprehensive stability data upon request. Every shipment includes a detailed Certificate of Analysis (COA) with batch‑specific HPLC purity, chromatographic traceability, and water content data — structured for direct insertion into your documentation systems and regulatory dossiers.

Flexible supply across all development scales. Cosperpharm supplies Fmoc-Thr-OH·H₂O across the full range of applications — from 1g–100g quantities for research and method development, to 500g–5kg batches for process validation and pilot production, to bulk quantities (up to 25kg or more) for commercial GMP peptide manufacturing. R&D samples typically ship within 5–7 business days; bulk orders ship within 2–3 weeks.

Technical support you can rely on. Our process chemistry team has extensive hands‑on experience in Fmoc SPPS and can advise on coupling condition optimization, resin selection, side‑chain protection strategies, and impurity profiling. We work alongside your team to ensure seamless scale‑up and effective troubleshooting across the entire peptide synthesis cascade.

Global logistics with transparent pricing. Cosperpharm ships to pharmaceutical manufacturers, CDMOs, CROs, and research institutions worldwide, with full customs documentation included in every shipment. Volume discounts apply at kilogram scales, and we communicate lead times clearly upfront — no hidden fees, no unexpected delays.

Product Advantages

1. Gold‑Standard Fmoc Protection for SPPS. Fmoc-Thr-OH·H₂O utilizes the Fmoc protection strategy — the preferred orthogonal protection system for modern SPPS. The Fmoc group is selectively cleaved under mild basic conditions (20% piperidine/DMF), a process that is rapid, clean, and fully orthogonal to acid‑labile side‑chain protecting groups. This enables the synthesis of complex, sensitive, and long peptide chains with minimal side reactions, making Fmoc-Thr-OH·H₂O the protected threonine derivative of choice for both research and GMP peptide manufacturing.

2. Free Side‑Chain Hydroxyl for Post‑Synthetic Modification. Unlike many other protected amino acids where side chains are blocked, Fmoc-Thr-OH·H₂O features a free β-hydroxyl group that remains unblocked throughout chain assembly. This free hydroxyl group serves as a direct handle for post‑synthetic functionalization — enabling O‑glycosylation for glycopeptide synthesis, O‑phosphorylation for studying cellular signaling pathways, and conjugation with fluorophores or biotin for peptide labeling and detection applications. The free hydroxyl group also contributes to the formation of hydrogen‑bonding networks critical for peptide secondary structure, particularly in β-sheet and turn motifs.

3. Monohydrate Crystalline Form for Superior Handling. The monohydrate form of Fmoc-Thr-OH·H₂O offers three critical practical advantages over the anhydrous form: enhanced crystallinity that prevents clumping and improves flow properties during weighing; superior long‑term storage stability under recommended conditions; and consistent, predictable performance in automated peptide synthesizers — all of which contribute to higher reproducibility and lower batch‑to‑batch variability in peptide synthesis campaigns.

4. Rigorous Analytical Quality Control. Cosperpharm supplies Fmoc-Thr-OH·H₂O at ≥98.0% HPLC purity as standard, with higher purity grades available upon request. Every batch is subject to multi‑technique characterization including HPLC (purity and enantiomeric purity), Karl Fischer water content analysis, ¹H NMR for structural confirmation, and specific optical rotation measurement to verify the correct stereochemistry — because your final peptide quality depends on the purity of every building block.

5. Broad Utility Across Therapeutic Peptide Platforms. Fmoc-Thr-OH·H₂O is the protected threonine derivative of choice for synthesizing a wide range of therapeutic peptides, peptide hormones, and glycopeptides — applications that span from drug discovery research to commercial GMP production of peptide pharmaceuticals.

6. Fully Compatible with Standard SPPS Protocols. Fmoc-Thr-OH·H₂O is compatible with all standard coupling reagents including HBTU/HOBt, HATU, PyBOP, and COMU, as well as all common SPPS resins. This plug‑and‑play compatibility streamlines process development and ensures seamless integration into existing synthesis workflows.

Product Quality Assurance

Cosperpharm has implemented a comprehensive quality assurance system for Fmoc-Thr-OH·H₂O that meets the rigorous expectations of both research‑scale discovery and GMP‑grade peptide manufacturing:

Thorough analytical characterization. Each batch of Fmoc-Thr-OH·H₂O undergoes multi‑technique analytical release testing: HPLC purity verification (≥98.0% sum of enantiomers, with full chromatographic traceability and enantiomeric purity confirmation), appearance verification (white to off‑white to pale yellow crystalline powder), water content determination by Karl Fischer, identity confirmation via ¹H NMR, specific optical rotation measurement to confirm stereochemical integrity ([α]²⁰/D −16±1°, c = 1% in DMF), and solubility verification in DMF. Physical property verification is conducted in accordance with established specifications.

Full batch traceability with retention samples. From raw material sourcing through synthesis, purification, recrystallization, drying, and final packaging, every step is fully documented and traceable. Each batch receives a unique lot number with sufficient retention samples maintained in accordance with Cosperpharm quality procedures — enabling complete backward traceability for regulatory purposes.

Stability monitoring program. Cosperpharm conducts ongoing stability studies under recommended storage conditions: long‑term stability monitoring at −20 °C under inert atmosphere, with accelerated stability studies conducted on representative batches. Stability summaries are updated regularly, with full data packages available to support customer regulatory submissions. Typical shelf life: 2–3 years at −20 °C; 6–12 months at 2–8 °C.

Documentation ready for regulatory submission. Every shipment includes a Certificate of Analysis (COA) with batch‑specific purity and characterization data, a Material Safety Data Sheet (SDS), and customs documentation for international delivery. For customers preparing ANDA or NDA filings for peptide drug substances, Cosperpharm provides DMF‑ready documentation packages, method validation reports, stability data, and custom quality agreements upon request. Traceability against USP, EP, or JP pharmacopeial standards can be provided based on feasibility.

Customer‑initiated quality audits. Qualified customers are welcome to audit Cosperpharm‘s manufacturing, quality control, and documentation facilities. Please contact our regulatory team to schedule an audit. ISO compliance documentation is available upon request.

Contact Us

Looking for a reliable source of Fmoc-Thr-OH·H₂O for your peptide synthesis campaign? Cosperpharm delivers high‑purity Fmoc‑protected threonine with the analytical documentation, supply flexibility, and technical support that pharmaceutical manufacturers and research institutions trust. Whether you need research quantities for method development, pilot batches for process validation, or bulk shipments for GMP manufacturing, our team is ready to provide detailed pricing, lead times, and documentation information. Contact us today to discuss your requirements.